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Automated cell counter with the option for a reusable hemocytometer and fluorescence capabilities With the option for a and fluorescence capabilities—brightfield and two user-changeable fluorescence channels—the Invitrogen Countess II FL Automated Cell Counter can count cells, monitor fluorescent protein expression, and measure cell viability. • Affordable—option for reusable slide to significantly reduce consumables cost • Accuracy—eliminate the subjectivity of manual cell counting and minimize user-to-user variability • Speed—count live and dead cells and measure viability and average cell size in as little as 10 seconds. Advanced autofocusing and counting algorithms The Countess automated cell counters contain advanced autofocusing and counting algorithms to allow you to quickly and accurately identify and count cells within a population (Figure 1). Countess instruments are compatible with a broad range of cell types and cell lines, and we have. Upon insertion of the slide the Countess instrument will automatically focus on cells, which helps to minimize user variation associated with manual focusing. • Download the application note. Time savings The additional time it takes to manually count cells using a hemocytometer (up to 5 minutes) compared to counting with the Countess II automated cell counters (10 seconds) is often overlooked as an added cost.
An individual counting five slides per day (two samples per slide) can save ~10 hours per month by switching to an automated counter with a reusable slide. The time savings increases to ~15 hours per month when using disposable slides (Figure 6). This additional time can be applied to other activities in the lab, resulting in a significant advantage when switching from manual to automated cell counting.
Full spectrum of fluorescence detection–not limited to GFP and RFP The Countess II FL instrument has two optional fluorescence channels. The fluorescent colors collected are determined by the insertion of individual light cubes. With 20 light cubes to choose from, the Countess II FL instrument is not limited to detection of GFP- and RFP-expressing cells. Looking to measure the transfection efficiency of cells transduced with an RFP-expression construct with a blue nuclear counterstain? Simply add the RFP and DAPI light cubes, and you can effectively count the number of cells expressing the RFP fusion within the total population and accurately determine the transfection efficiency. Gating based on fluorescence intensity The ability to use up to two customizable fluorescence colors with the Countess II FL Automated Cell Counter allows for more accurate counts when dealing with complex samples that contain significant amounts of platelets, RBCs, or debris.
In addition, the ability to gate based on fluorescence intensity to determine how many cells are dim vs. Bright, or are expressing a fluorescent protein, can save time and effort prior to downstream analysis with techniques such as flow cytometry or high-content analysis ( Figure 7). Technical specifications • Processing time. The result, image, and data files can be easily exported to a USB drive. The result and image files can be saved as.bmp,. Imran Hashmi Songs Mp3 Download Free. jpg,.tiff, or.png files.
The data file is saved as a.csv file that can be easily imported into your current analysis software or viewed in Excel™ software. A comma-separated values (CSV) file stores tabular data (numbers and text) in plaintext form. Plain text means that the file is a sequence of characters, with no data that has to be interpreted as binary numbers. A CSV file can be opened with any third party software or spreadsheet program.
The table below describes the categories of the Countess II data saved as a CSV file and opened with a spreadsheet program. Technical specifications • Processing time. The result, image, and data files can be easily exported to a USB drive. The result and image files can be saved as.bmp,.jpg,.tiff, or.png files. The data file is saved as a.csv file that can be easily imported into your current analysis software or viewed in Excel™ software. A comma-separated values (CSV) file stores tabular data (numbers and text) in plaintext form. Plain text means that the file is a sequence of characters, with no data that has to be interpreted as binary numbers.
A CSV file can be opened with any third party software or spreadsheet program. The table below describes the categories of the Countess II data saved as a CSV file and opened with a spreadsheet program. Install Openfiler On Usb Flash.