Cytokine Cell Biology: A Practical Approach 0 1 2 3 4 rd Edition 0 1 2 3 4 0 edited by Fran Balkwill 1 Cytokine Molecular Biology: A Practical Approach, 3rd Edition 2 A. Kristina Downing Wellcome Trust Senior Research Fellow, OCMS and Department of Biochemistry, University of Oxford, South Parks Rd, OX1 3QH, UK 3 Journal of Cell Science, Bidder Building, 140 Cowley Rd, Cambridge, UK CB4 0DL 4 Ron Jacob Centre for Cardiovascular Biology and Medicine, King's College London, Guy's Campus, London, SE1 1UL, UK Cytokines are important in the regulation of the immune system, not only controlling growth and differentiation but providing a messaging network through which different components of the immune system can interact.

Over the past decade, the number of identified cytokines has expanded enormously - the interleukins alone now number over 20 members. Following the recent publication of the human genome sequence, the number of known cytokines will surely increase dramatically over the next few years. Given the importance of cytokines in the immune system, many of these molecules are being investigated as potential therapeutic targets, and this field of study can only expand in the future. D Link Webcam Installation Drivers. An updated 'practical approach to cytokine biology' therefore arrives at an opportune moment. - The latest edition has been split into 2 volumes, Cytokine Cell Biology and Cytokine Molecular Biology. Cytokine Cell Biology covers the techniques necessary to investigate the production and activity of cytokines. Each chapter, either directly or through reference to other chapters, covers the methods required to perform experiments from the initial preparation of cells through to the protocols for assaying cytokine function.

Further information about the conditions required for successful cell culture and techniques such as FACS analysis, are also clearly presented, enabling complex experiments to be designed from start to finish. Chapters including detailed assays for the effect of cytokines on the activation, proliferation, migration and cytotoxicity of cells are included, with specific reference to macrophages, B and T lymphocytes. In addition to the study of cytokine function, several chapters deal with methods for the detection of cytokine production. These include protocols for immunohistochemical analysis, ELISA, Elispot and FACSbased assays of cytokines. In areas such as this, it would be beneficial to have access to both the cellular and molecular biology volumes, because the molecular volume contains an excellent chapter detailing additional methods such as RNase protection and RT-PCR for assaying cytokine gene expression. Cytokine Molecular Biology focuses mainly on the study of cytokine signalling.

Several sections describe the expression and purification of cytokine proteins and their use in receptor binding studies. Further chapters provide a broad spectrum of techniques for the study of cytokine receptor signal transduction. The activity of signalling molecules often correlates with their phosphorylation status, and protocols to study the phosphorylation status of signalling molecules such as MAP kinases are detailed. Alternative strategies, such as directly assaying kinase activity with known substrates, the use of chemical inhibitors and the study of transcription factor activation, are also described, with the pros and cons of each method clearly explained. Additional information about the study of lipid-based signal transduction completes a thorough explanation of the techniques required for the study of cytokine signal transduction. Cytokine Molecular Biology finishes with a wellwritten chapter on the use of transgenesis in the study of cytokines. This chapter details the standard methods for generating transgenic animals, including knockout and conditional knockout techniques.

Introduction to Protein Science: Architecture, Function, and Genomics (2nd edition). Author: Arthur M. Edition, the new edition integrates treatment of bioinformatics, databases and computational methods of determining and predicting protein structure and function, and.Missing. Mation now available, to help determine protein structure and function. The chapter ends. ©2004 New Science Press Ltd From Sequence to Function Chapter 4 131.

For a more detailed description of the contents of the two books check the publishers website at www.oup.co.uk. In general, both volumes are well written and clearly laid out, with just the right amount of experimental detail and background information. The books do not merely present basic protocols but provide additional information on reagents, equipment and often handy tips with respect to particular reagents or the design of experimental controls. The chapters with which we were already well versed appeared to be complete and well explained. Other chapters on techniques with which we were not familiar proved to be easily accessible, with respect to both the protocols and the background information behind the techniques and the science. We would recommend both of these books to anyone working in the field of cytokines or immunology, and feel buying both together would provide a complete coverage of most of the commonly used techniques in immunology.

These books would be extremely useful for both PhD students and postdocs alike - although at 30 each, students may prefer to recommend them to their libraries. In general, if signalling is your thing go for Cytokine Molecular Biology, for the effects of cytokines on cellular behaviour get Cytokine Cell Biology, and if possible get them both. A guide for the New Age traveller in protein Introduction to Protein Architecture: the structural biology of proteins by Arthur M. Lesk Before reading this book, I would never have believed that a close examination of protein structure could be so animated. Lesk has taken this subject and brought it to life. The book is ideal for readers who have a relatively strong background knowledge of biochemistry but little or no understanding of how protein structure relates to function. In his Preface, Lesk quotes Rutherford, who said, All science is either physics or stamp collecting.

He then explains, This book embodies my reply, that the study of proteins includes the best qualities of both. This is an accurate appraisal of the text. The underlying physics of protein architecture is covered in sufficient detail for one to gain an appreciation of proteins as micro-machines that function mechanically. At the same time, the book is beautifully illustrated with countless colour figures that impress the variety and elegant complexity of protein structure.

On the first page of the Contents section, there is a note stating Sections marked with an asterisk may be skipped on a first reading. Then, out of more than 100 sections, only 7 are marked, comprising about 20 of the 300+ pages. Having read this book, Im sure that the author had a very hard time suggesting that any section could be skipped! His passion for protein architecture emanates from the text, and his enthusiasm will no doubt be infectious for those wishing to learn more about the subject. Lesks literary style makes for easy reading. The text follows a logical organisation in which the readers interest is stimulated first by introduction of the photosynthetic reaction centre from Rhodopseudomonas viridis, which illustrates all the essential principles of protein conformation. He invites, Let us look at it and take it apart.

Next, there is a description of protein sequence and how 3D structures are derived experimentally. Principles of secondary (helix and sheet) and tertiary structure follow, with extensive reference to classifications of protein structures - for example, in the databases SCOP (and CATH (cath/). Molecular evolution is considered in different contexts and on different time scales. The complicated relationships between amino acid sequence similarity and structural homology with convergent versus divergent evolution are presented in detail. The evolution of function within specific protein families is then highlighted, followed by an introduction to the proteins of the immune system, and the amazingly sophisticated and rapid maturation of the antibody response.

Finally, functional aspects of conformational change in proteins are explored. Throughout the book Lesk keeps his sense of humour, and the readers interest, with a few well-placed quips, such as Rotation of the actin during the activity of the [ATPase] was seen and recorded. This movie certainly deserves an Oscar in the natural history category, staring ATPase (and with some fine actin in the cast). Problem sections at the end of each chapter are comprehensive and innovative.

Bosch Power Mixx 800w User Guide. In particular, this book is timely in its use of electronic material found on the World Wide Web. A number of the problems (weblems, as they are referred to) require the interactive use of web databases and/or software. However, one drawback of this text is that there are no problem solutions provided. Understanding how protein function follows form will be one of the great challenges of the 21st century.

With the recent boom in sequence data, scientists are now preparing to map out all of protein structure space experimentally. As we seek to navigate the trails of this new universe, Lesks book will provide a handy map and a useful guide for many travellers. Calcium cuisine Calcium Signalling, 2nd Edition, A Practical Approach edited by Alexei V.

Tepikin I have fond memories of the first edition of this book. Alas, they are only memories because, as an anonymous tribute to its usefulness, someone purloined it from my office. However, its loss is now mitigated by the publication of a worthy successor that has all the attributes of a good cookery book, with many well written chapters that include recipes (protocols) and colour plates to whet the appetite. Being greedy I would have liked more, and this volume is rather slim for the price compared with others in the OUP Practical Approach series. The book is divided into three parts, the first containing four chapters covering the techniques of calcium measurement, including the recently developed targeted chimeric cameleons and aequorins. The now conventional fluorescent indicators do not warrant a chapter of their own but are covered implicitly in other chapters, including one specifically devoted to discussing artefacts arising from the presence of transition metals and zinc. Most of the book concentrates on the biological aspects of calcium measurement, and rightly so, but the technical aspect of fluorescence measurement gets its own chapter covering photometry, video imaging, and confocal and multiphoton microscopy.

At 25 pages long, this chapter can give only a brief introduction. Although most chapters include some discussion of the instrumentation required, readers looking for in-depth information about instrumentation will need to look elsewhere.

The second part covers calcium measurement in different organelles, with chapters on endoplasmic recticulum and the nucleoplasm and its envelope, and an excellent chapter from Michael Duchens laboratory on mitochondria, which includes the associated measurement of membrane potential and redox state. Taken in conjunction with the relevant sections on targeted proteins from the first part, the discussion of the rapidly developing field of organellar calcium measurement is one of the main strengths of this book.

The third part comprises four chapters on the monitoring of specific calcium reactions. I suspect this name was invented to avoid a section labelled, chapters that do not fit in to the other two categories but nonetheless, it covers important and interesting topics. The first chapter is on controlling cytoplasmic calcium and measuring calcium-dependent gene expression. Here, Dolmetsch and Negulescu describe, in some detail, their calcium clamp apparatus that has been used so successfully to investigate the effect of calcium oscillations on gene expression. The second chapter is from Andrew Thomas laboratory, and describes how to measure calcium in an intact liver. This is followed by a chapter from Antony Galiones laboratory on the use of sea urchin egg homogenates to investigate calcium release mechanisms.

The final chapter is from the Editor, Alexei Tepikin, on the droplet technique for measuring calcium extrusion from single cells. One topic that I feel should have been included is the use of caged compounds, a technique that is a natural accompaniment to the fluorescent measurement of calcium, and that has been used successfully to control cytoplasmic calcium. Like the calcium clamp it has provided important data on the calcium regulation of gene expression, and yet it barely warrants a mention. However, despite this reservation, Tepikin and OUP are to be congratulated on bringing together a distinguished collection of world authorities and persuading them to write interesting and authoritative articles. I certainly picked up new ideas, and this is a book that will be compulsory reading for graduate students and postdocs wishing to cook in my calcium kitchen.

I am sure that any calcium restaurant successfully using the full gamut of these recipes would warrant three Michelin stars. JCS Commentaries highlight and critically discuss recent exciting work that will interest those working in cell biology, molecular biology, genetics and related disciplines. These short reviews are commissioned from leading figures in the field and are subject to rigorous peer-review and in-house editorial appraisal. Each issue of the journal contains at least two Commentaries. JCS thus provides readers with more than fifty Commentaries over the year, which cover the complete spectrum of cell science. Although we discourage submission of completed unsolicited Commentaries to the journal, ideas for future articles in the form of a short proposal and some key references are welcome and should be sent to the Executive Editor at the address below.

Introduction to protein science architecture function and genomics pdf • 1. Introduction to Protein Science: Architecture, Function, and Genomics Arthur M. Lesk • Publisher: Oxford University Press Release Date: • ISBN: Author: Arthur M. Lesk Download Here • Designed for students in biology, biochemistry, and biomedicine, Introduction to Protein Science, Second Edition, provides a wide-ranging introduction to the contemporary study of proteins in health and disease. The text describes basic principles of protein structure and methods for studying them, illustrates the wide variety of functions that proteins have, and shows how their structures and functions are closely linked. Introduction to Protein Science, Second Edition, relates the study of proteins to the context of modern high-throughput data streams of genomics and proteomics.

It also provides a balanced treatment of the relationship between computational and experimental methods. The text is enhanced by marginal notes, exercises, problems, and 'weblems' that develop students' database and computational server skills. An updated and expanded Companion Website offers resources for students and instructors. Find the Full PDF Here • You Can Download the PDF Here Powered by TCPDF (www.tcpdf.org).